#MILLIPORE QUICKSCALE 70 X 550 MANUAL#

Add distilled water or buffer to 4 times the resin volume and stir thoroughly. Once suspended, transfer the required amount of suspension (approximately 4 volumes suspension = 3 volumes resin) into a container of sufficient volume to hold 4 times the volume of resin being prepared. a) The settled resin in the shipping containers should be suspended by vigorous agitation or stirring with a rod or paddle (do not use a magnetic stirrer it will grind the resin, generating fines).

The following decantation process is required to remove fines from the resin slurry. Fines in the gel slurry may obstruct screens or sintered filters and may eventually increase the pressure drop across the column. FIGURE 1 Equipment required for packing 1.2 Removal of Fines Tosoh Bioscience recommends that fines be removed. 1) required to successfully pack resins are: a pump, a pressure gauge, a level, glasses, acrylic or PEEK or stainless steel column and a packing reservoir (optional). Although it is not recommended, resins can be packed by simple gravitational settling. Preparation for Packing 1.1 General Considerations for Packing It is best to pack resins by the application of pressure from 0.05 to 0.3 MPa across the bed length. It also confers upon the resin excellent pressure/flow characteristics and ph stability. These resins are a modified methacrylate polymer which gives the resin a hydrophilic surface due to the presence of ether and hydroxyl groups.

They are applicable for the laboratory and process scale purifications of globular proteins, peptides, nucleic acids, and other biologically derived materials. Process Column Installations 16Ĥ 2 PACKING Introduction chromatographic resins are macroporous polymeric packings for bioprocess chromatography. Column Operation Chromatographic Separation Size Exclusion (SEC) Ion Exchange (IEC) Hydrophobic Interaction (HIC) Affinity (AFC) Cleaning Storage Sterilization/Depyrogenation/ Preservative Removal/Column Frits PressureFlow Curves and Performance Testing Results for Selected Resins. Equilibration and Efficiency Evaluation 8 4. Packing Procedure Constant Velocity / Semiconstant Pressure Method 6 3. Preparation for Packing General Consideration for Packing Removal of Fines Buffer Equilibration Slurry Preparation Alternative Slurry Preparation 4 2.

#MILLIPORE QUICKSCALE 70 X 550 MANUAL#

63 MARKET STREET #1003 BANK OF SINGAPORE CENTRE SINGAPORE, SINGAPORE T F TOSOH HISTORY 1935 FOUNDING OF TOYO SODA MANUFACTURING CO., LTD OPERATION OF NANYO MANUFACTURING COMPLEX BEGINS 1971 SCIENTIFIC INSTRUMENTS DIVISION FORMED, FIRST GPC COLUMN USING TSKgel DEVELOPED BY TOSOH 1974 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN PLANT IS COMPLETED 1979 TOSOH DEVELOPS MEDIA 1983 TOSOH DEVELOPS HYDROPHOBIC INTERACTION MEDIA 1987 TOSOHAAS US OPERATIONS FORMED IN MONTGOMERYVILLE 1989 TOSOHAAS GMBH OPERATIONS FORMED IN STUTTGART 1995 TOSOH NANYO GEL FACILITY RECEIVES ISO /2003 ALL TOSOH AFFILIATED SCIENTIFIC & DIAGNOSTIC SYSTEM RELATED COMPANIES IN EUROPE ARE UNIFIED UNDER THE NAME TOSOH BIOSCIENCE EcoSEC, THE 7TH GENERATION GPC SYSTEM IS INTRODUCED GLOBALLY 2010 TOSOH CELEBRATES ITS 75TH YEAR IN BUSINESS WITH THE OPENING OF FIVE NEW PLANTS, AND CONTINUED RAPID EXPANSION IN CHINA 2011 TOSOH BIOSCIENCE CELEBRATES 40 YEARS OF OPERATION 2012 TOSOH RELEASES FIRST MIXEDMODE RESIN MXTrp650M 2013 TOSOH RELEASES A HIGH CAPACITY PROTEIN A CHROMATOGRAPHY RESIN 2014 TOSOH BIOSCIENCE GMBH CELEBRATES ITS 25 TH ANNIVERSARY IN STUTTGART 2015 TOSOH BIOSCIENCE SUCCESSFULLY MOVES ITS SALES & MARKETING OFFICES TO GRIESHEIM, DARMSTADTģ TOSOH BIOSCIENCE PROCESS 1 Instruction Manual Table of Contents I. ROOM 1001, INNOV TOWER, BLOCK A, NO HONGMEI ROAD XU HUI DISTRICT SHANGHAI,, CHINA T F 5 TOSOH ASIA PTE. 2 1 TOSOH BIOSCIENCE GMBH IM LEUSCHNERPARK GRIESHEIM GERMANY T + 49 (0) F + 49 (0) 2 TOSOH BIOSCIENCE LLC 3604 HORIZON DRIVE, SUITE 100 KING OF PRUSSIA, PA 19406, USA T F 3 TOSOH CORPORATION 382 SHIBA, MINATOKU TOKYO JAPAN T F TOSOH BIOSCIENCE SHANGHAI CO.

Millipore quickscale 70 x 550